All-in-one mRNA production and purification kit
Bench scale, for research use only
The Ntensify mano is an all-in-one, cost-effective mRNA production and purification kit combining convenience, efficiency, and control. The pack includes all reagents needed to produce up to 3 mg of purified mRNA in less than half a day.
The mRNA production kit performs the Ntensify process for cost-effective production of purified mRNA, with a high-quality profile while minimizing impurities.
Tailored for the Ntensify process
Redesigned Process
By opting for a single-step bead-based purification method to streamline DSP and reduce process-related losses.
Optimized Process
By optimizing the IVT & DSP to increase RNA quality, minimizing the DSP constraints.
Demonstrated by robust DoE
- > 2000 individual mRNA reactions
- 100+ different constructs tested
- Different mRNA sizes (1 to 4kb)
Up to
3 mg
of purified mRNA
From linear DNA to purified mRNA in under
3 hours
1 pot IVT
with co-transcriptional capping
1-step
proprietary silica bead-based purification
Supplied with regular and modified
uridines
The kit contains 8 mixes, , including cap-analog, uridine, pseudo-uridine, and methyl pseudo-uridine. mRNA can be produced in less than half a day and uses standard lab equipment such as a thermo-shaker, a vortex and a benchtop centrifuge making it accessible to researchers of all experience levels.
Refer to the picture below or watch our video presentation to discover what’s inside the box!
Visualization of Ntensify mano components and process: integrated IVT and purification.
Best purified using our Ntensify manomag
The Ntensify manomag is finely calibrated for optimal retention of the magnetic beads, enhancing purification efficiency and mRNA yield. This ensures consistently high-quality mRNA production.
Easy-to-use protocol
The Ntensify mano mRNA production kit also contains an easy-to-use protocol, designed for user convenience. All reagent mixes are optimized to work together, enabling high mRNA yield while minimizing process-related impurities. Additionally, we deliver the mixes in compact boxes with clear labeling on each cap, further easing handling operations.
Adopted by
CNC – University of Coimbra Portugal used the Ntensify mano kit and approves its quality!
“We are happy to inform you that the mRNA synthesized with your Ntensify mano kit was as good as the quality from CDMOs within the industry.”
“Using the Ntensify mano, we achieved low impurity levels and excellent in vivo efficiency with bispecific antibody-encoded mRNA for colorectal cancer treatment in mice.”
All-in-one saRNA production and purification kit
Bench scale, for research use only
The Ntensify mano is an all-in-one, cost-effective saRNA production and purification kit combining convenience, efficiency, and control. The pack includes all reagents needed to produce up to 3.2 mg of purified saRNA in less than half a day.
The saRNA production kit performs the Ntensify process for cost-effective production of purified saRNA, with a high-quality profile while minimizing impurities.
Tailored for the Ntensify process
Redesigned Process
By opting for a single-step bead-based purification method to streamline DSP and reduce process-related losses.
Optimized Process
By optimizing the IVT & DSP to increase RNA quality, minimizing the DSP constraints.
Demonstrated by robust DoE
- > 500 individual saRNA reactions
- 100+ different constructs tested
- Different saRNA sizes (8 to 12kb)
Up to
3.2 mg
of purified saRNA
From linear DNA to purified saRNA in under
3 hours
1 pot IVT
with co-transcriptional capping
1-step
magnetic silica bead-based purification
We further developed our Ntensify mRNA process for its use on saRNA
Study design
- 8 saRNA constructs (duplicated)
- 2 independent experiments
- Construct size: 8–12 kb
Key improvements
- 8 saRNA constructs (duplicated)
- 2 independent experiments
- Construct size: 8–12 kb
Results showing consistent IVT titers averaging 8-12g/L, across a variety of constructs and a broad range of construct lengths
Results showing consistent integrity measurements averaging 81.9% , across a variety of constructs and a broad range of construct lengths
Source: DOI: 10.1042/bio_2021_142
Beyond mRNA, saRNA further increase vaccines affordability and efficac
saRNA facilitates robust antigen expression at lower doses thanks to the self-replicating properties, thereby enhancing both immunogenicity and cost-effectiveness
BENEFITS
- Enables RNA self-amplification, reducing required dose.
- Potential for durable immunity.
- Allows rapid, cell-free, and scalable production (good for pandemic response).
- Increases vaccine yield from each manufacturing batch.
- Enhances delivery efficiency and protects RNA from degradation.
Compared to mRNA, saRNA provides a longer-lasting expression
The kit contains 8 mixes, saRNA can be produced in less than half a day and uses standard lab equipment such as a thermo-shaker, a vortex and a benchtop centrifuge making it accessible to researchers of all experience levels.
Refer to the picture to discover what’s inside the box!
Visualization of Ntensify mano components and process: integrated IVT and purification.
Best purified using our Ntensify manomag
The Ntensify manomag is finely calibrated for optimal retention of the magnetic beads, enhancing purification efficiency and mRNA yield. This ensures consistently high-quality mRNA production.
Easy-to-use protocol
The Ntensify mano mRNA production kit also contains an easy-to-use protocol, designed for user convenience. All reagent mixes are optimized to work together, enabling high mRNA yield while minimizing process-related impurities. Additionally, we deliver the mixes in compact boxes with clear labeling on each cap, further easing handling operations.