All-in-one mRNA production kit
Bench scale, for research use only
The Ntensify mano is an all-in-one, cost-effective mRNA production kit combining convenience, efficiency, and control. It includes all reagents needed to produce up to 1 mg of purified mRNA in less than half a day.
This mRNA production kit performs the Ntensify process for cost-effective production of purified mRNA, with a high-quality profile while minimizing impurities.
Tailored for the Ntensify process
Redesigned Process
By opting for a single-step bead-based purification method to streamline DSP and reduce process-related losses.
Optimized Process
By optimizing the IVT & DSP to increase RNA quality, minimizing the DSP constraints.
Demonstrated by robust DoE
- > 2000 individual mRNA reactions
- > 500 individual saRNA reactions
- 100+ different constructs tested
- Different mRNA sizes (1 to 4kb)
- Different saRNA sizes (8 to 12kb)
Up to
1 mg
of purified mRNA
From linear DNA to purified mRNA in under
3 hours
1 pot IVT
with co-transcriptional capping
1-step
proprietary silica bead-based purification
Supplied with regular and modified
uridines
The kit contains 8 mixes, , including cap-analog, uridine, pseudo-uridine, and methyl pseudo-uridine. mRNA can be produced in less than half a day and uses standard lab equipment such as a thermo-shaker, a vortex and a benchtop centrifuge making it accessible to researchers of all experience levels.
Refer to the picture below or watch our video presentation to discover what’s inside the box!
Visualization of Ntensify mano components and process: integrated IVT and purification.
Best purified using our Ntensify manomag
The Ntensify manomag is finely calibrated for optimal retention of the magnetic beads, enhancing purification efficiency and mRNA yield. This ensures consistently high-quality mRNA production.
Easy-to-use protocol
The Ntensify mano mRNA production kit also contains an easy-to-use protocol, designed for user convenience. All reagent mixes are optimized to work together, enabling high mRNA yield while minimizing process-related impurities. Additionally, we deliver the mixes in compact boxes with clear labeling on each cap, further easing handling operations.
Adopted by
CNC – University of Coimbra Portugal used the Ntensify mano kit and approves its quality!
“We are happy to inform you that the mRNA synthesized with your Ntensify mano kit was as good as the quality from CDMOs within the industry.”
“Using the Ntensify mano, we achieved low impurity levels and excellent in vivo efficiency with bispecific antibody-encoded mRNA for colorectal cancer treatment in mice.”