Ntensify™ mano

All-in-one mRNA production and purification kit

Bench scale, for research use only

The Ntensify® mano is an all-in-one, cost-effective mRNA production and purification kit combining convenience, efficiency and control. The pack includes all reagents needed to produce up to 3 mg of purified mRNA in less than 3 hours.

The mRNA production performs the Ntensify® process for cost-effective production of purified mRNA, with a high-quality profile while minimizing impurities.

1 kit for 990 €/$
Ntensify® mano mRNA kit
▶ Watch the video presentation

Tailored for the Ntensify® process

Conventional process
IVT
Post transcriptional capping
Oligo DT
Polish
UF/DF
Ntensify® process
IVT with co-transcriptional capping
Silica-based magnetic beads
Redesigned Process

By opting for a single-bead-based purification method to streamline DSP and reduce process-related losses.

Optimized Process

By optimizing the IVT & DSP to increase RNA quality, minimizing the DSP constraints.

Demonstrated by robust DoE
  • ✓2000+ individual mRNA reactions
  • ✓100+ different constructs tested
  • ✓Different mRNA sizes (1 to 4kb)
Average Ntensify® process performance — mRNA (Version 4.5)
Up to 3 mg
of purified mRNA
3 hours
purified mRNA in under 3 hours
1 pot IVT
with co-transcription capping
1-step
purification bead-based

The kit contains 8 mixes, including cap-analog, uridine, pseudo-uridine, and methyl pseudo-uridine. mRNA can be produced in less than 3 hours and uses standard lab equipment such as a thermo-shaker, a vortex and a benchtop centrifuge, making it accessible to researchers of all experience levels.

Refer to the picture below or watch our video presentation to discover what's inside the box.

IVT
RNA transcription
Purification
RNA purification
Components
Control DNA
Mix 1a
UTP
Ψ-UTP
m1-Ψ-UTP
Mix 2
Mix 3
Mix 4
Mix 5
Mix 6
BB
NFW
Labels
Storage box
-20°C
RT

Visualization of Ntensify® mano components and process: integrated IVT and purification.

Ntensify® manomag magnetic bead rack

Best purified using our Ntensify® manomag

The Ntensify® manomag is finely calibrated for optimal retention of the magnetic beads, enhancing purification efficiency and RNA yield. This ensures consistently high-quality mRNA production.

Easy-to-use protocol

The Ntensify® mano production kit also contains an easy-to-use protocol designed for user convenience. All reagent mixes are optimized to work together, enabling high RNA yield while minimizing process-related impurities. Additionally, we deliver the mixes in compact boxes with clear labeling on each cap, further easing handling operations.

Ntensify® mano easy-to-use protocol manual
Adopted by
CNC

CNC – University of Coimbra Portugal used the Ntensify® mano kit and approves its quality!

UC Berkeley

"We are happy to inform you that the mRNA synthesized with your Ntensify® mano kit was as good as the quality from CDMOs within the industry."

University of Copenhagen

"Using the Ntensify® mano, we achieved low impurity levels and excellent in-vivo efficiency with bispecific antibody-encoded mRNA for colorectal cancer treatment in mice."

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