Nplify™

We have the solution for cell-free linear DNA supply

Our Ntensify™ users require rapid access to a safe and clean DNA supply as a starting material for RNA production. We provide a DNA on-demand service that offers cell-free linear DNA production in less than 6 weeks starting from an in-silico DNA sequence.

The service delivers the most suitable DNA for mRNA synthesis with no additional linearization steps required  - ready to use in your Ntensify™ system.

white-down-grey

Choose the service that meets your needs:

pDNA template assembly
  • In-silico design of the plasmid DNA

  • Synthesis of the gene of interest sequence

  • Vector cloning and purification

  • Screening of clones by sequencing

  • QC of the plasmid DNA

  • Research cell bank of the plasmid DNA

Output is a DNA template as starting material for Ntensify™ Mini for use in Drug Discovery applications

Cell-Free Linear DNA Production & QC
  • quantoom-icon

    High-throughput production of cell-free linear DNA with the Nplify™  system (PCR-based amplification)

  • quantoom-icon

    High-throughput analytical testing to ensure product specifications are met

  • quantoom-icon

    Cell-free linear DNA product with a full batch validation report

Primer design, synthesis & PCR conditions screening
  • Design and synthesis of primer candidates at small scale

  • Screening and determination of the best PCR conditions with primer candidate pairs

  • Synthesis of the best pair of primers identified at a larger scale

Output is a DNA template as starting material for the Ntensify™ Mini and the Ntensify™ Midi for use in Pre-clinical applications

Cell-Free Linear DNA Production & QC
  • quantoom-icon

    High-throughput production of cell-free linear DNA with the Nplify™  system (PCR-based amplification)

  • quantoom-icon

    High-throughput analytical testing to ensure product specifications are met

  • quantoom-icon

    Cell-free linear DNA product with a full batch validation report

This service is provided thanks to our proprietary benchtop system, NplifyTM, suitable for cell-free enzymatic production of linear DNA from a plasmid or gene of interest. The system is fully automated and integrates both PCR-based amplification and purification steps. It was designed to produce linear DNA for the subsequent manufacture of RNA using our Ntensify system.

If you have questions or would like to learn more about Quantoom’s DNA manufacturing services, please contact us.

Contact Us

Please complete the form below for any commercial, partnering, media, press, or general inquiries.

If you need to contact our customer service team, please click on the button below.

Quantoom
black-up-orange