Nplify™
We have the solution for cell-free linear DNA supply
Our Ntensify™ users require rapid access to a safe and clean DNA supply as a starting material for RNA production. We provide a DNA on-demand service that offers cell-free linear DNA production in less than 6 weeks starting from an in-silico DNA sequence.
The service delivers the most suitable DNA for mRNA synthesis with no additional linearization steps required - ready to use in your Ntensify™ system.
Choose the service that meets your needs:
pDNA template assembly
In-silico design of the plasmid DNA
Synthesis of the gene of interest sequence
Vector cloning and purification
Screening of clones by sequencing
QC of the plasmid DNA
Research cell bank of the plasmid DNA
Output is a DNA template as starting material for Ntensify™ Mini for use in Drug Discovery applications
Cell-Free Linear DNA Production & QC
High-throughput production of cell-free linear DNA with the Nplify™ system (PCR-based amplification)
High-throughput analytical testing to ensure product specifications are met
Cell-free linear DNA product with a full batch validation report
Primer design, synthesis & PCR conditions screening
Design and synthesis of primer candidates at small scale
Screening and determination of the best PCR conditions with primer candidate pairs
Synthesis of the best pair of primers identified at a larger scale
Output is a DNA template as starting material for the Ntensify™ Mini and the Ntensify™ Midi for use in Pre-clinical applications
Cell-Free Linear DNA Production & QC
High-throughput production of cell-free linear DNA with the Nplify™ system (PCR-based amplification)
High-throughput analytical testing to ensure product specifications are met
Cell-free linear DNA product with a full batch validation report
This service is provided thanks to our proprietary benchtop system, NplifyTM, suitable for cell-free enzymatic production of linear DNA from a plasmid or gene of interest. The system is fully automated and integrates both PCR-based amplification and purification steps. It was designed to produce linear DNA for the subsequent manufacture of RNA using our Ntensify system.
If you have questions or would like to learn more about Quantoom’s DNA manufacturing services, please contact us.
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